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ezrA [2017-8-11 16:0:3]
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ezrA [2017-8-11 16:0:3]

negative regulator of FtsZ ring formation
locus
BSU29610
pI
4.76
mw
64.82 kDa
protein length
562 aa Sequence Blast
gene length
1686 bp Sequence Blast
function
control of FtsZ ring formation
product
FtsZ-interacting protein
essential
no
synonyms
ytwP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.8|Cell division] → [category|SW 1.1.8.2|Other genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,029,729 → 3,031,417

    Phenotypes of a mutant

  • altered localization of [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] and [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]]] [Pubmed|25403286]
  • growth defects, elongated cells [Pubmed|25403286]
  • the ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' mutation is synthetically lethal with a ''[gene|DB09F1C36257F511A84A083967A25A9D46744D14|sepF]'' mutation [Pubmed|24218584]
  • a ''[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA] [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' double mutant grows poorly, and the cells are filamentous [Pubmed|24097947]
  • a ''[gene|EAD44AA7D72510B70308FD0C9F159DBEDAEBBB74|zapA] [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' double mutant forms filamentous cells [Pubmed|16420366]
  • depletion of ''[gene|953DE0F0B81894ECFF4C0693511AC238BF3D0C0A|pdhA]'' and deletion of ''[gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]'' have a strong synthetic defect in [SW|cell division] [Pubmed|24825009]

    • The protein

    Catalyzed reaction/ biological activity

  • inhibits [protein|41872E2EF00C79918DD077F2EF78F37E24FEB110|FtsZ] polymerization [Pubmed|25403286]
  • recruits [gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]]] to the division septum [Pubmed|25403286]
  • activates [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC] [Pubmed|25845974]

    • Protein family

  • ezrA family (according to Swiss-Prot)

    • Effectors of protein activity

  • degradation of [protein|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|EzrA] involves [protein|4E7B9426CED372AA8A321A147116A3A589FBF20C|FtsH] [Pubmed|24222488]

    • Structure

  • [PDB|4UXV] (the 60 kDa cytoplasmic domain) [Pubmed|25403286]

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]
  • 1 transmembrane domain at the N-terminus [Pubmed|25403286]

    • Expression and Regulation

    Operons

    genes
    [gene|B317D7E51824DD70EF84E4D5D7290D601BF4FAB6|ezrA]
    description
    [Pubmed|9387221]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|15317798], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulation

  • constitutively expressed [Pubmed|23701187]

    • additional information

  • degradation of [protein|search|EzrA] involves [protein|search|FtsH] [PubMed|24222488]
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    Biological materials

    Mutant

  • MGNA-A428 (ytwP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/428 NBRP B. subtilis, Japan]
  • 1A805 ( ''ezrA''::''kan''), [Pubmed|15101985], available at [http://bgsc.org BGSC]
  • 1A1279 (''ezrA''::''spec''), [Pubmed|10449747], available at [http://bgsc.org BGSC]
  • 1A1280 (''ezrA-gfp''), [Pubmed|10449747], available at [http://bgsc.org BGSC]

    • References

    Reviews

  • 19680248,19884039,28357277

  • Original Publications

  • 10449747,16420366,15317782,16549676,17662947,12368265,18363795,9387221,15317798,19429628,18763711,21401734,24222488,23701187,17718511,24218584,24097947,24825009,25068683,25403286,25845974,25954268